Technically no, but a dog the character forms a connection with dies emotionally and many lab animals die. Main character is also a vet and talks about having to put down peoples pets in the past
A Guinea pig has its genitals mutilated by another, and on page 262 it’s briefly mentioned that a bee is “birthed” from someone’s scrotum through a large welt/hole
While not explicitly called an eating disorder, the mother's behavior lines up pretty well with binge eating disorder. This character is portrayed as a monster, especially in relation to her increasing size. Some of the prose is extremely derogatory towards people in larger bodies.
A mother rapes one of her sons on at least one occasion. She's also described crawling into the other son's bed. Throughout the story in general, sexual abuse between the mother and her sons is implied and later made explicit in one flashback scene