Father Gascoigne goes mad during the Hunt, and his wife leaves her daughter to find him. You can speak to her through the window at her house, and she asks you to find her parents.
Not overtly, but there are quite a lot of dogs in cages that were left outside on the night that the game takes place on, something that led to those dogs turning into extremely hostile beasts.
The game is extremely violent and several characters die regardless of gender. Women are not specifically singled out, but the overall atmosphere is grim and brutal. Gender, femininity, menstruation, and pregnancy are all major underlying themes. Potentially triggering events (spoilers):
-The Queen of Yharnam is depicted with her wrists bound and wearing a wedding dress. She is pregnant and covered in blood near her womb.
-A female character is a prostitute. If she survives until the game's third act, she gives birth to an eldritch creature.
There's several eldritch alien babies you can kill, though they don't really look like babies.
Especially not the Orphan of Kos, who is newborn but also looks like an old man.
The boss Rom the Vacuous Spider is a huge, spider-like Great Old One. She also periodically summons a horde of spiders. These appear as regular enemies in the Nightmare of Mensis area.
The word "Rape" is never explicitly mentioned, but there is a note saying that a "womb will be blessed with child." The woman to whom this is referring does not seem have had any say in the matter.
Women are unwillingly impregnated by Eldritch Abominations. This usually happens by magic shennanigans instead of direct assault, but I'd say unwilling impregnation counts as sexual assault.
Of particular note is the DLC boss fight against Ludwig the Accursed/Holy Blade. After his defeat, his head is severed (though he remains alive after that until someone, either you or a different NPC, finishes him off.)
One version of the bloodletting beast, found in the Lower Pthumeru Chalice (Layer 4) and the Great Pthumeru Ihyll Chalice (Layer 2) is headless, and the muscle inside of its neck is visible.
If the player is killed by a certain enemy during a certain part of the game, it is implied that they are actually knocked unconscious. During a scene from the player's POV, they awaken while being dragged in a cloth bag to a new area.
Beasts with multiple/too many eyes are fought commonly late in the game, and though there is no detailed eye gore, this can still be upsetting to somebody with trypophobia or a fear of things with too many eyes.
Discussed. Mergo, the child of Queen Yharnam, is said to have been "ripped from her womb," and there are implications that they were either miscarried or stolen by the Old Ones, but it is never clarified.
While the previous comment is correct with regard to the fate of Father Gascoigne's daughters, it is incorrect with regard to their names. Neither girl has a canonical name. "Violet" is likely derived from the canonical name of their mother, Viola (found on the brooch that the younger daughter requests for you to find and return) and "Adelle" would be immensely confusing given that there is another NPC named Adella.
Father Gascoigne, called that in reference to the fact that he has two daughters, is a required boss under normal circumstances. Neither of his daughters are named.
Furthermore, his wife (who does have a name, and it is Viola) is found dead after you are tasked to look for her by one of their daughters.
The entire game is triggering. The whole of the what area is teeth itchingly bad but there are a dozen or more monsters and sequences that either rub up against trypophobia or outright are there to trigger. Miyazaki gravitated towards trypophobia triggers for squick factor in all of his games.
You may come across an npc eating corpses in the forbidden woods. Also in the fishing hamlet there are giant whale enemies that have an attack which will swallow your character whole.
Technically, yes; if you die to an enemy called a Snatcher, you wake up in the Hypogean Gaol and have to fight (or sneak) your way back to a lantern. While the Snatchers are far from law enforcement, the Gaol is very much a jail in every sense of the word, even if it is a poorly maintained one.
Additionally, an NPC you can meet in the Gaol implies that 1) she had been there for a very long time 2) there were others taken with her that aren't around any longer and likely died there.
More than an attempt, if Father Gascoigne's daughter is sent to either the Clinic or the Chapel, her older sister will throw herself from a ledge when she finds out her family is all dead.
The sounds from the blood and gore could trigger someone and I think some enemies can be heard eating corpses? There's also an enemy with a machine gun that makes a really grating noise
Hoo boy, strap in everyone...
Most enemies are mutated by the Scourge, a werewolf epidemic. One variant of werewolf is mutated to the point that it has exposed, rotten organs and a lower leg replacing its tail.
The Cramped Casket enemy is an appartment buildings worth of corpses all smooshed together from stuffing them into the same coffin and leaving them to rot in the street.
One enemy has its head mounted sideways and is infested with tapeworms.
The boss Brain of Mensis is a giant, undead brain studded with eyes.
On that note, "insight" is implied to grow eyes on the surface of the PC's brain. It's a pun you see...
The boss The One Reborn is a heap of fused, undead corpses used in a failed ritual intended to elevate mankind into Godhood, but resulted in, well, a heap of fused, undead corpses.
One boss is fought 3 times, becoming ever more decrepit as you do so. In the last fight, it's head has fallen off and been replaced by a parasitic worm.
The chalice dungeons tend to be closed in and, in some places, tightly packed. Fortunately, the dungeons are entirely avoidable and are used mostly for grinding.
In the Old Hunter's DLC, a character named Lady Maria of the Astral Clocktower is shown slumped in a chair with a slit throat, though she does grab your wrist a begin speaking to you when you approach her. As you find her in an alternate plane of reality where several confirmed dead characters are "alive" and trapped as a form of punishment, it is heavily implied she killed herself only to become trapped also (possibly intentionally).
Well, the Old Ones' inability to bear or beget viable offspring is a huge plot point, and the final boss is the undead remains of their latest attempt.
Weeeeellllll... the baby is a perfectly healthy starborne horror whose birth drove their mother gigglingly bonkers, but it's not a miscarriage... it is, however, vastly accelerated, taking about 6 hours from conception to birth.
Always off-screen but Arianna gives birth to an Eldritch baby if she survives long enough, and it is implied several women in the game get pregnant, including one you run into two or three times as an "apparition" of sorts.
Not technically a peroson, but Kos, an Eldritch extraplaner being known as a "Great One," died for unknown reasons while pregnant, leading to a postmortem birth.
"Fatty" is one of the words players can use to write messages, and can frequently be found next to passages that are too narrow for the player's character to enter.
A character who is an old man in present day is shown to have had inappropriate obsessive feelings, most likely of a romantic nature, for a character who was his student when she was a young adult. Given that she has been dead for many years and he has an unnaturally prolonged lifespan, we do not know their relative ages during the period of their (one-sided) "relationship", but the visual ages of the character models could be seen as having a substantial age difference.
A substantial part of the game surrounds a ministration called The Healing Church, with many themes and titles referencing a traditional Christian church
Given the nature of the Beast Plague, it could be presumed that any of the beasts you fight on the streets of Yharnam are terminally ill, but of particular note is Gilbert, a man you only speak to through a window near the beginning of the game. His coughing is loud enough to be heard from the nearby lantern, and he is quite polite and helpful to the player. Unfortunately, regardless of the player's actions, Gilbert eventually succumbs to the plague himself and transforms into a beast that is as hostile to you as to any other.
Blood and gore are big plot elements. Blood from wounds, blood that heals, blood that freezes into jewels, blood that never clots offered in sacrifice.